Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) of Macular Pigment
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime imaging ophthalmoscopy
Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can...
متن کاملTomographic fluorescence lifetime imaging
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution lifetime images of thin tissue sections. This chapter concerns the in vivo time domain imaging of fluorescent contrast agents embedded in de...
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We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the relaxation rate of the fluorophores. The tech...
متن کاملFluorescence ophthalmoscopy.
FLUORESCENCE ophthalmoscopy is an important manoeuvre that can provide a great deal of information about certain pathological lesions of the retina and choroid, but simple observation of the fundus appearances after intravenous fluorescein injection has tended to be neglected in favour of photographic recordings. The apparatus required for the latter is expensive and time-consuming, and often r...
متن کاملTwo-photon microscopy and fluorescence lifetime imaging of retinal pigment epithelial cells under oxidative stress.
PURPOSE The aim of this study was to investigate the autofluorescence (AF) of the RPE with two-photon microscopy (TPM) and fluorescence lifetime imaging (FLIM) under normal and oxidative stress conditions. METHODS Porcine RPE-choroid explants were used for investigation. The RPE-choroid tissue was preserved in a perfusion organ culture system. Oxidative stress was induced by laser photocoagul...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2018
ISSN: 1552-5783
DOI: 10.1167/iovs.18-23886